Description
Induced by specific ligands, soybean agglutinin (SBA) spontaneously assembles into highly uniform protein microtubes. However, current Cryo-EM techniques can only capture the static, “dead” structures of the final product. How these micro-tubes actually nucleate and grow in an aqueous environment remains a mystery. To crack this, we developed a closed Flow Cell system coupled with TIRF-STORM super-resolution imaging, successfully capturing the in-situ dynamic assembly of microtubes at the single-molecule level.
Primary author
晨 徐
(复旦大学)
